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The Infectious Clone Construction of Tomato Yellow Leaf Curl Virus Isolate from TianjinKeywords: Tomato yellow leaf curl virus , infectious clone , Tianjin isolate construction Abstract: Tomato Yellow Leaf Curl Disease (TYLCD) is a serious disease that affects tomato production worldwide. Breeding resistant cultivar is an effective method to avoid this disease. In the program of breeding, the inoculation method to identify the plant resistance is particularly important. In this study, the infectious clone of TYLCV Tianjin isolate (TYLCV-TJ) was constructed. The inoculation test showed the infectious clone was effective in inoculated Solanum lycopersicum, typical downward yellow leaf curl was observed at 30 dpi.
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